Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3328294..3329069 Replicon chromosome
Accession NZ_CP021718
Organism Klebsiella pneumoniae strain AR_0129

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag AM376_RS19305 Protein ID WP_004150910.1
Coordinates 3328294..3328779 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag AM376_RS19310 Protein ID WP_004150912.1
Coordinates 3328776..3329069 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM376_RS19275 3323463..3324830 - 1368 WP_004150905.1 replicative DNA helicase -
AM376_RS19280 3324823..3325206 - 384 WP_004150906.1 hypothetical protein -
AM376_RS19285 3325206..3326078 - 873 WP_004188557.1 ParA family protein -
AM376_RS19295 3326684..3326900 - 217 Protein_3238 transposase -
AM376_RS19300 3326997..3327590 - 594 WP_004188553.1 hypothetical protein -
AM376_RS19305 3328294..3328779 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
AM376_RS19310 3328776..3329069 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
AM376_RS19315 3329714..3331090 + 1377 WP_004217775.1 cystathionine beta-synthase -
AM376_RS19320 3331101..3332249 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
AM376_RS19325 3332250..3333161 - 912 WP_004188550.1 LysR family transcriptional regulator -
AM376_RS19330 3333259..3333861 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3271274..3329320 58046
flank IS/Tn - - 3326748..3326900 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T77115 WP_004150910.1 NZ_CP021718:c3328779-3328294 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T77115 NZ_CP021718:c3328779-3328294 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT77115 WP_004150912.1 NZ_CP021718:c3329069-3328776 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT77115 NZ_CP021718:c3329069-3328776 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References