Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737410..738185 Replicon chromosome
Accession NZ_CP068610
Organism Klebsiella pneumoniae strain 45706

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JMZ75_RS03680 Protein ID WP_004150910.1
Coordinates 737700..738185 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JMZ75_RS03675 Protein ID WP_004150912.1
Coordinates 737410..737703 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ75_RS03655 732618..733220 - 603 WP_002916735.1 short chain dehydrogenase -
JMZ75_RS03660 733318..734229 + 912 WP_004188550.1 LysR family transcriptional regulator -
JMZ75_RS03665 734230..735378 - 1149 WP_087760405.1 PLP-dependent aspartate aminotransferase family protein -
JMZ75_RS03670 735389..736765 - 1377 WP_165758780.1 cystathionine beta-synthase -
JMZ75_RS03675 737410..737703 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JMZ75_RS03680 737700..738185 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JMZ75_RS03685 738889..739482 + 594 WP_004188553.1 hypothetical protein -
JMZ75_RS03690 739579..739795 + 217 Protein_725 transposase -
JMZ75_RS03695 740401..741273 + 873 WP_004188557.1 ParA family protein -
JMZ75_RS03700 741273..741656 + 384 WP_004150906.1 hypothetical protein -
JMZ75_RS03705 741649..743016 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739579..739731 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T187391 WP_004150910.1 NZ_CP068610:737700-738185 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T187391 NZ_CP089792:2058789-2058892 [Salmonella enterica subsp. enterica serovar Kentucky]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT187391 WP_004150912.1 NZ_CP068610:737410-737703 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT187391 NZ_CP089792:c2058894-2058751 [Salmonella enterica subsp. enterica serovar Kentucky]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References