69989

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4719110..4719885 Replicon chromosome
Accession NZ_CP018437
Organism Klebsiella pneumoniae strain MNCRE53

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag BTE50_RS26750 Protein ID WP_004150910.1
Coordinates 4719110..4719595 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag BTE50_RS26755 Protein ID WP_004150912.1
Coordinates 4719592..4719885 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTE50_RS26720 4714279..4715646 - 1368 WP_004150905.1 replicative DNA helicase -
BTE50_RS26725 4715639..4716022 - 384 WP_004150906.1 hypothetical protein -
BTE50_RS26730 4716022..4716894 - 873 WP_004188557.1 ParA family protein -
BTE50_RS26740 4717500..4717716 - 217 Protein_4527 transposase -
BTE50_RS26745 4717813..4718406 - 594 WP_004188553.1 hypothetical protein -
BTE50_RS26750 4719110..4719595 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
BTE50_RS26755 4719592..4719885 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
BTE50_RS26760 4720530..4721906 + 1377 WP_004217775.1 cystathionine beta-synthase -
BTE50_RS26765 4721917..4723065 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
BTE50_RS26770 4723066..4723977 - 912 WP_004188550.1 LysR family transcriptional regulator -
BTE50_RS26775 4724075..4724677 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4662089..4720136 58047
flank IS/Tn - - 4717564..4717716 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(41-143)

Antitoxin

(3-95)


Sequences


Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T69989 WP_004150910.1 NZ_CP018437:c4719595-4719110 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T69989 NZ_CP018437:c4719595-4719110 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT69989 WP_004150912.1 NZ_CP018437:c4719885-4719592 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT69989 NZ_CP018437:c4719885-4719592 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References