Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742605..743380 Replicon chromosome
Accession NZ_CP087639
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02391

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LMH57_RS03710 Protein ID WP_004150910.1
Coordinates 742895..743380 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LMH57_RS03705 Protein ID WP_004150912.1
Coordinates 742605..742898 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH57_RS03685 (LMH57_03685) 737813..738415 - 603 WP_062954968.1 short chain dehydrogenase -
LMH57_RS03690 (LMH57_03690) 738513..739424 + 912 WP_004188550.1 LysR family transcriptional regulator -
LMH57_RS03695 (LMH57_03695) 739425..740573 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LMH57_RS03700 (LMH57_03700) 740584..741960 - 1377 WP_004217775.1 cystathionine beta-synthase -
LMH57_RS03705 (LMH57_03705) 742605..742898 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LMH57_RS03710 (LMH57_03710) 742895..743380 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LMH57_RS03715 (LMH57_03715) 744084..744677 + 594 WP_004188553.1 hypothetical protein -
LMH57_RS03720 (LMH57_03720) 744774..744990 + 217 Protein_731 transposase -
LMH57_RS03725 (LMH57_03725) 745596..746468 + 873 WP_004188557.1 ParA family protein -
LMH57_RS03730 (LMH57_03730) 746468..746851 + 384 WP_004150906.1 hypothetical protein -
LMH57_RS03735 (LMH57_03735) 746844..748211 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744774..744926 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225783 WP_004150910.1 NZ_CP087639:742895-743380 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225783 NZ_CP121356:666028-666342 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225783 WP_004150912.1 NZ_CP087639:742605-742898 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225783 NZ_CP121356:666339-666755 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References