Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 797143..797918 Replicon chromosome
Accession NZ_CP056425
Organism Klebsiella pneumoniae strain RHBSTW-00515

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag HV227_RS04045 Protein ID WP_048265986.1
Coordinates 797433..797918 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HV227_RS04040 Protein ID WP_004150912.1
Coordinates 797143..797436 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV227_RS04020 792352..792954 - 603 WP_048265987.1 short chain dehydrogenase -
HV227_RS04025 793052..793963 + 912 WP_014906929.1 LysR family transcriptional regulator -
HV227_RS04030 793964..795112 - 1149 WP_020316731.1 PLP-dependent aspartate aminotransferase family protein -
HV227_RS04035 795123..796499 - 1377 WP_074189249.1 cystathionine beta-synthase -
HV227_RS04040 797143..797436 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HV227_RS04045 797433..797918 + 486 WP_048265986.1 GNAT family N-acetyltransferase Toxin
HV227_RS04050 798623..799216 + 594 WP_048265985.1 hypothetical protein -
HV227_RS04055 799313..799529 + 217 Protein_797 transposase -
HV227_RS04065 800207..800920 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 799313..799465 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17623.66 Da        Isoelectric Point: 8.5144

>T163459 WP_048265986.1 NZ_CP056425:797433-797918 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTVPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T163459 NZ_CP072586:190004-190270 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT163459 WP_004150912.1 NZ_CP056425:797143-797436 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT163459 NZ_CP072586:189741-190004 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References