Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 714855..715630 Replicon chromosome
Accession NZ_CP087629
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02341

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LMH53_RS03555 Protein ID WP_004150910.1
Coordinates 715145..715630 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LMH53_RS03550 Protein ID WP_004150912.1
Coordinates 714855..715148 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH53_RS03530 (LMH53_03530) 710063..710665 - 603 WP_062954968.1 short chain dehydrogenase -
LMH53_RS03535 (LMH53_03535) 710763..711674 + 912 WP_004188550.1 LysR family transcriptional regulator -
LMH53_RS03540 (LMH53_03540) 711675..712823 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LMH53_RS03545 (LMH53_03545) 712834..714210 - 1377 WP_004217775.1 cystathionine beta-synthase -
LMH53_RS03550 (LMH53_03550) 714855..715148 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LMH53_RS03555 (LMH53_03555) 715145..715630 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LMH53_RS03560 (LMH53_03560) 716334..716927 + 594 WP_004188553.1 hypothetical protein -
LMH53_RS03565 (LMH53_03565) 717024..717240 + 217 Protein_700 transposase -
LMH53_RS03570 (LMH53_03570) 717846..718718 + 873 WP_004188557.1 ParA family protein -
LMH53_RS03575 (LMH53_03575) 718718..719101 + 384 WP_004150906.1 hypothetical protein -
LMH53_RS03580 (LMH53_03580) 719094..720461 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 717024..717176 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225731 WP_004150910.1 NZ_CP087629:715145-715630 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225731 NZ_CP121296:3454686-3454789 [Salmonella enterica subsp. enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225731 WP_004150912.1 NZ_CP087629:714855-715148 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225731 NZ_CP121296:c3454791-3454648 [Salmonella enterica subsp. enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References