Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4330188..4330963 Replicon chromosome
Accession NZ_CP028542
Organism Klebsiella pneumoniae strain WCHKP2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CAY63_RS23495 Protein ID WP_004150910.1
Coordinates 4330188..4330673 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CAY63_RS23500 Protein ID WP_004150912.1
Coordinates 4330670..4330963 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY63_RS23465 4325520..4326887 - 1368 WP_004150905.1 replicative DNA helicase -
CAY63_RS23470 4326880..4327263 - 384 WP_004150906.1 hypothetical protein -
CAY63_RS23475 4327263..4328135 - 873 WP_004188557.1 ParA family protein -
CAY63_RS23480 4328245..4328430 - 186 WP_085763813.1 hypothetical protein -
CAY63_RS23485 4328578..4328794 - 217 Protein_4332 IS3 family transposase -
CAY63_RS23490 4328891..4329484 - 594 WP_004188553.1 hypothetical protein -
CAY63_RS23495 4330188..4330673 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CAY63_RS23500 4330670..4330963 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CAY63_RS23505 4331614..4332984 + 1371 WP_004150913.1 cystathionine beta-synthase -
CAY63_RS23510 4332995..4334143 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CAY63_RS23515 4334144..4335055 - 912 WP_004188550.1 LysR family transcriptional regulator -
CAY63_RS23520 4335153..4335755 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4272131..4331214 59083
flank IS/Tn - - 4328642..4328794 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T101399 WP_004150910.1 NZ_CP028542:c4330673-4330188 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T101399 NZ_CP028542:c4330673-4330188 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT101399 WP_004150912.1 NZ_CP028542:c4330963-4330670 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT101399 NZ_CP028542:c4330963-4330670 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References