Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 744266..745041 Replicon chromosome
Accession NZ_CP087657
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01635

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LMH51_RS03685 Protein ID WP_004150910.1
Coordinates 744556..745041 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LMH51_RS03680 Protein ID WP_004150912.1
Coordinates 744266..744559 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH51_RS03660 (LMH51_03660) 739474..740076 - 603 WP_062954968.1 short chain dehydrogenase -
LMH51_RS03665 (LMH51_03665) 740174..741085 + 912 WP_004188550.1 LysR family transcriptional regulator -
LMH51_RS03670 (LMH51_03670) 741086..742234 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LMH51_RS03675 (LMH51_03675) 742245..743621 - 1377 WP_004217775.1 cystathionine beta-synthase -
LMH51_RS03680 (LMH51_03680) 744266..744559 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LMH51_RS03685 (LMH51_03685) 744556..745041 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LMH51_RS03690 (LMH51_03690) 745745..746338 + 594 WP_004188553.1 hypothetical protein -
LMH51_RS03695 (LMH51_03695) 746435..746651 + 217 Protein_726 transposase -
LMH51_RS03700 (LMH51_03700) 747257..748129 + 873 WP_004188557.1 ParA family protein -
LMH51_RS03705 (LMH51_03705) 748129..748512 + 384 WP_004150906.1 hypothetical protein -
LMH51_RS03710 (LMH51_03710) 748505..749872 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 746435..746587 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225861 WP_004150910.1 NZ_CP087657:744556-745041 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225861 NZ_CP121483:2481876-2482085 [Staphylococcus epidermidis]
ATGAAATTAAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225861 WP_004150912.1 NZ_CP087657:744266-744559 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225861 NZ_CP121483:2481576-2481869 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References