Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4074987..4075762 Replicon chromosome
Accession NZ_CP090061
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP-NE368

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LRM38_RS20310 Protein ID WP_004150910.1
Coordinates 4075277..4075762 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LRM38_RS20305 Protein ID WP_004150912.1
Coordinates 4074987..4075280 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM38_RS20285 (LRM38_20270) 4070195..4070797 - 603 WP_002916735.1 short chain dehydrogenase -
LRM38_RS20290 (LRM38_20275) 4070895..4071806 + 912 WP_004188550.1 LysR family transcriptional regulator -
LRM38_RS20295 (LRM38_20280) 4071807..4072955 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LRM38_RS20300 (LRM38_20285) 4072966..4074342 - 1377 WP_004217775.1 cystathionine beta-synthase -
LRM38_RS20305 (LRM38_20290) 4074987..4075280 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LRM38_RS20310 (LRM38_20295) 4075277..4075762 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LRM38_RS20315 (LRM38_20300) 4076466..4077059 + 594 WP_004188553.1 hypothetical protein -
LRM38_RS20320 (LRM38_20305) 4077156..4077372 + 217 Protein_3973 transposase -
LRM38_RS20325 (LRM38_20310) 4077978..4078850 + 873 WP_004188557.1 ParA family protein -
LRM38_RS20330 (LRM38_20315) 4078850..4079233 + 384 WP_004150906.1 hypothetical protein -
LRM38_RS20335 (LRM38_20320) 4079226..4080593 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4077156..4077308 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T229971 WP_004150910.1 NZ_CP090061:4075277-4075762 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T229971 NZ_CP124652:1402083-1402373 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT229971 WP_004150912.1 NZ_CP090061:4074987-4075280 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT229971 NZ_CP124652:1401787-1402086 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References