Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 836850..837625 Replicon chromosome
Accession NZ_CP103567
Organism Klebsiella pneumoniae strain 4680

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag M5S58_RS04175 Protein ID WP_021314147.1
Coordinates 837140..837625 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag M5S58_RS04170 Protein ID WP_004150912.1
Coordinates 836850..837143 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S58_RS04150 (832058) 832058..832660 - 603 WP_021440720.1 short chain dehydrogenase -
M5S58_RS04155 (832758) 832758..833669 + 912 WP_048290040.1 LysR family transcriptional regulator -
M5S58_RS04160 (833670) 833670..834818 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
M5S58_RS04165 (834829) 834829..836205 - 1377 WP_004217775.1 cystathionine beta-synthase -
M5S58_RS04170 (836850) 836850..837143 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
M5S58_RS04175 (837140) 837140..837625 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
M5S58_RS04180 (838329) 838329..838922 + 594 WP_004188553.1 hypothetical protein -
M5S58_RS04185 (839019) 839019..839235 + 217 Protein_823 transposase -
M5S58_RS04190 (840198) 840198..841058 + 861 WP_000158645.1 DUF6387 family protein -
M5S58_RS04195 (841274) 841274..841522 + 249 WP_000616796.1 AlpA family transcriptional regulator -
M5S58_RS04200 (841519) 841519..841830 + 312 WP_000837631.1 hypothetical protein -
M5S58_RS04205 (841845) 841845..842231 + 387 WP_001545719.1 hypothetical protein -
M5S58_RS04210 (842248) 842248..842535 + 288 WP_001291073.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 839019..839171 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T255594 WP_021314147.1 NZ_CP103567:837140-837625 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT255594 WP_004150912.1 NZ_CP103567:836850-837143 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References