Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5066185..5066960 Replicon chromosome
Accession NZ_CP050371
Organism Klebsiella pneumoniae strain 50595

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HB637_RS25430 Protein ID WP_004150910.1
Coordinates 5066185..5066670 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HB637_RS25435 Protein ID WP_004150912.1
Coordinates 5066667..5066960 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB637_RS25405 5061354..5062721 - 1368 WP_004150905.1 replicative DNA helicase -
HB637_RS25410 5062714..5063097 - 384 WP_004150906.1 hypothetical protein -
HB637_RS25415 5063097..5063969 - 873 WP_004188557.1 ParA family protein -
HB637_RS25420 5064575..5064791 - 217 Protein_4847 transposase -
HB637_RS25425 5064888..5065481 - 594 WP_004188553.1 hypothetical protein -
HB637_RS25430 5066185..5066670 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HB637_RS25435 5066667..5066960 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HB637_RS25440 5067605..5068981 + 1377 WP_004217775.1 cystathionine beta-synthase -
HB637_RS25445 5068992..5070140 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HB637_RS25450 5070141..5071052 - 912 WP_004188550.1 LysR family transcriptional regulator -
HB637_RS25455 5071150..5071752 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 5008387..5067211 58824
flank IS/Tn - - 5064639..5064791 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T152752 WP_004150910.1 NZ_CP050371:c5066670-5066185 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T152752 NZ_CP066492:1881589-1881684 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT152752 WP_004150912.1 NZ_CP050371:c5066960-5066667 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT152752 NZ_CP066492:c1881769-1881712 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References