Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 734282..735057 Replicon chromosome
Accession NZ_CP096238
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02201

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag M0536_RS03635 Protein ID WP_004889762.1
Coordinates 734572..735057 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag M0536_RS03630 Protein ID WP_004150912.1
Coordinates 734282..734575 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0536_RS03610 (729491) 729491..730093 - 603 WP_002916735.1 short chain dehydrogenase -
M0536_RS03615 (730191) 730191..731102 + 912 WP_014906929.1 LysR family transcriptional regulator -
M0536_RS03620 (731103) 731103..732251 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
M0536_RS03625 (732262) 732262..733638 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
M0536_RS03630 (734282) 734282..734575 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
M0536_RS03635 (734572) 734572..735057 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
M0536_RS03640 (735761) 735761..736354 + 594 WP_004188553.1 hypothetical protein -
M0536_RS03645 (736451) 736451..736667 + 217 Protein_717 transposase -
M0536_RS03655 (737345) 737345..738058 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 736451..736603 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T242937 WP_004889762.1 NZ_CP096238:734572-735057 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T242937 NZ_OW968111:3742143-3742421 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT242937 WP_004150912.1 NZ_CP096238:734282-734575 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT242937 NZ_OW968111:3741880-3742140 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References