Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4309946..4310721 Replicon chromosome
Accession NZ_CP066543
Organism Klebsiella pneumoniae strain BTS

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag D6T75_RS22165 Protein ID WP_004150910.1
Coordinates 4309946..4310431 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag D6T75_RS22170 Protein ID WP_004150912.1
Coordinates 4310428..4310721 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T75_RS22140 4305115..4306482 - 1368 WP_004150905.1 replicative DNA helicase -
D6T75_RS22145 4306475..4306858 - 384 WP_004150906.1 hypothetical protein -
D6T75_RS22150 4306858..4307730 - 873 WP_004188557.1 ParA family protein -
D6T75_RS22155 4308336..4308552 - 217 Protein_4123 transposase -
D6T75_RS22160 4308649..4309242 - 594 WP_200302495.1 hypothetical protein -
D6T75_RS22165 4309946..4310431 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
D6T75_RS22170 4310428..4310721 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
D6T75_RS22175 4311366..4312742 + 1377 WP_200302496.1 cystathionine beta-synthase -
D6T75_RS22180 4312753..4313901 + 1149 WP_200302497.1 PLP-dependent aspartate aminotransferase family protein -
D6T75_RS22185 4313902..4314813 - 912 WP_004188550.1 LysR family transcriptional regulator -
D6T75_RS22190 4314911..4315513 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4251621..4310972 59351
flank IS/Tn - - 4308400..4308552 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T184631 WP_004150910.1 NZ_CP066543:c4310431-4309946 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T184631 NZ_CP087706:c4638488-4638156 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGACGGCGAAGCTATGTCTGACGTCCGCGGATACCTGGCAAATGTTACTGAC
ACGTCTGCTAGAACAGCATTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCCCTCTGGCCGATGCAGTTAACTTTCTGGTGGATAAGTACGCACTGGTTCGTATCGATCGCAGAGGATTG
AGTTGGCAGGAGCAATCATCCTATCTCCGGCTTGTCGATACTCAGCGAACCATTAAGGTTATTGAGTTGTGGCTATTTGG
TGTAATGATGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT184631 WP_004150912.1 NZ_CP066543:c4310721-4310428 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT184631 NZ_CP087706:c4638826-4638509 [Klebsiella pneumoniae]
ATGAGCAACATCACTTGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGATCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGTTGACCACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTCCGGCTACGTATACATCGCTGTTTATCTCACCCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References