Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2191668..2192443 Replicon chromosome
Accession NZ_AP024756
Organism Klebsiella pneumoniae strain TUM12128

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6J96_RS10795 Protein ID WP_004150910.1
Coordinates 2191668..2192153 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6J96_RS10800 Protein ID WP_004150912.1
Coordinates 2192150..2192443 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J96_RS10770 (TUM12128_20560) 2186837..2188204 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
K6J96_RS10775 (TUM12128_20570) 2188197..2188580 - 384 WP_004150906.1 hypothetical protein -
K6J96_RS10780 (TUM12128_20580) 2188580..2189452 - 873 WP_004188557.1 ParA family protein -
K6J96_RS10785 2190058..2190274 - 217 Protein_2116 transposase -
K6J96_RS10790 (TUM12128_20590) 2190371..2190964 - 594 WP_004188553.1 hypothetical protein -
K6J96_RS10795 (TUM12128_20610) 2191668..2192153 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6J96_RS10800 (TUM12128_20620) 2192150..2192443 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6J96_RS10805 (TUM12128_20630) 2193088..2194464 + 1377 WP_004217775.1 cystathionine beta-synthase -
K6J96_RS10810 (TUM12128_20640) 2194475..2195623 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6J96_RS10815 (TUM12128_20650) 2195624..2196535 - 912 WP_004188550.1 LysR family transcriptional regulator -
K6J96_RS10820 (TUM12128_20660) 2196633..2197235 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2133447..2192694 59247
flank IS/Tn - - 2190122..2190274 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T39475 WP_004150910.1 NZ_AP024756:c2192153-2191668 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T39475 NZ_AP024756:c2192153-2191668 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT39475 WP_004150912.1 NZ_AP024756:c2192443-2192150 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT39475 NZ_AP024756:c2192443-2192150 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References