Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 740429..741204 Replicon chromosome
Accession NZ_CP110967
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-424/19

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NPX97_RS03695 Protein ID WP_004150910.1
Coordinates 740719..741204 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NPX97_RS03690 Protein ID WP_004150912.1
Coordinates 740429..740722 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX97_RS03670 (NPX97_03670) 735637..736239 - 603 WP_002916735.1 short chain dehydrogenase -
NPX97_RS03675 (NPX97_03675) 736337..737248 + 912 WP_004188550.1 LysR family transcriptional regulator -
NPX97_RS03680 (NPX97_03680) 737249..738397 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NPX97_RS03685 (NPX97_03685) 738408..739784 - 1377 WP_004217775.1 cystathionine beta-synthase -
NPX97_RS03690 (NPX97_03690) 740429..740722 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NPX97_RS03695 (NPX97_03695) 740719..741204 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NPX97_RS03700 (NPX97_03700) 741908..742501 + 594 WP_004188553.1 hypothetical protein -
NPX97_RS03705 (NPX97_03705) 742598..742814 + 217 Protein_729 transposase -
NPX97_RS03710 (NPX97_03710) 743420..744292 + 873 WP_004188557.1 ParA family protein -
NPX97_RS03715 (NPX97_03715) 744292..744675 + 384 WP_004150906.1 hypothetical protein -
NPX97_RS03720 (NPX97_03720) 744668..746035 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742598..742750 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T264623 WP_004150910.1 NZ_CP110967:740719-741204 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT264623 WP_004150912.1 NZ_CP110967:740429-740722 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References