Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3629425..3630200 Replicon chromosome
Accession NZ_CP040038
Organism Klebsiella pneumoniae strain KPC160125

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag FDK27_RS18530 Protein ID WP_004150910.1
Coordinates 3629425..3629910 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FDK27_RS18535 Protein ID WP_004150912.1
Coordinates 3629907..3630200 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDK27_RS18505 3624594..3625961 - 1368 WP_004150905.1 replicative DNA helicase -
FDK27_RS18510 3625954..3626337 - 384 WP_004150906.1 hypothetical protein -
FDK27_RS18515 3626337..3627209 - 873 WP_004188557.1 ParA family protein -
FDK27_RS18520 3627815..3628031 - 217 Protein_3555 transposase -
FDK27_RS18525 3628128..3628721 - 594 WP_004188553.1 hypothetical protein -
FDK27_RS18530 3629425..3629910 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
FDK27_RS18535 3629907..3630200 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FDK27_RS18540 3630845..3632221 + 1377 WP_004217775.1 cystathionine beta-synthase -
FDK27_RS18545 3632232..3633380 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
FDK27_RS18550 3633381..3634293 - 913 Protein_3561 LysR family transcriptional regulator -
FDK27_RS18555 3634391..3634993 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3572404..3630451 58047
flank IS/Tn - - 3627879..3628031 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T124832 WP_004150910.1 NZ_CP040038:c3629910-3629425 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T124832 NZ_CP040038:c3629910-3629425 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT124832 WP_004150912.1 NZ_CP040038:c3630200-3629907 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT124832 NZ_CP040038:c3630200-3629907 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References