Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 756115..756890 Replicon chromosome
Accession NZ_CP065949
Organism Klebsiella pneumoniae strain KP2509

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JDE60_RS03805 Protein ID WP_004150910.1
Coordinates 756405..756890 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JDE60_RS03800 Protein ID WP_004150912.1
Coordinates 756115..756408 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDE60_RS03780 (JDE60_03780) 751323..751925 - 603 WP_062954968.1 short chain dehydrogenase -
JDE60_RS03785 (JDE60_03785) 752023..752934 + 912 WP_004188550.1 LysR family transcriptional regulator -
JDE60_RS03790 (JDE60_03790) 752935..754083 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
JDE60_RS03795 (JDE60_03795) 754094..755470 - 1377 WP_004217775.1 cystathionine beta-synthase -
JDE60_RS03800 (JDE60_03800) 756115..756408 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JDE60_RS03805 (JDE60_03805) 756405..756890 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JDE60_RS03810 (JDE60_03810) 757594..758187 + 594 WP_004188553.1 hypothetical protein -
JDE60_RS03815 (JDE60_03815) 758284..758500 + 217 Protein_750 transposase -
JDE60_RS03820 (JDE60_03820) 759106..759978 + 873 WP_004188557.1 ParA family protein -
JDE60_RS03825 (JDE60_03825) 759978..760361 + 384 WP_004150906.1 hypothetical protein -
JDE60_RS03830 (JDE60_03830) 760354..761721 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 758284..758436 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T183667 WP_004150910.1 NZ_CP065949:756405-756890 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T183667 NZ_CP087147:19053-19469 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT183667 WP_004150912.1 NZ_CP065949:756115-756408 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT183667 NZ_CP087147:18826-19056 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References