Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3359482..3360257 Replicon chromosome
Accession NZ_CP083445
Organism Klebsiella pneumoniae strain KP294

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LAV94_RS16460 Protein ID WP_004150910.1
Coordinates 3359772..3360257 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LAV94_RS16455 Protein ID WP_004150912.1
Coordinates 3359482..3359775 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAV94_RS16435 (3354690) 3354690..3355292 - 603 WP_002916735.1 short chain dehydrogenase -
LAV94_RS16440 (3355390) 3355390..3356301 + 912 WP_004188550.1 LysR family transcriptional regulator -
LAV94_RS16445 (3356302) 3356302..3357450 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LAV94_RS16450 (3357461) 3357461..3358837 - 1377 WP_004217775.1 cystathionine beta-synthase -
LAV94_RS16455 (3359482) 3359482..3359775 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LAV94_RS16460 (3359772) 3359772..3360257 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LAV94_RS16465 (3360961) 3360961..3361554 + 594 WP_004188553.1 hypothetical protein -
LAV94_RS16470 (3361651) 3361651..3361867 + 217 Protein_3210 transposase -
LAV94_RS16480 (3362545) 3362545..3363258 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3361651..3361803 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T216455 WP_004150910.1 NZ_CP083445:3359772-3360257 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T216455 NZ_CP113051:c1757297-1756935 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAATATAAATTGGATAAAGACTCAGTCATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTATTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT216455 WP_004150912.1 NZ_CP083445:3359482-3359775 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT216455 NZ_CP113051:c1757464-1757294 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAATCATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References