Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 716577..717352 Replicon chromosome
Accession NZ_CP089877
Organism Klebsiella pneumoniae strain KP308

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag LVQ70_RS03550 Protein ID WP_009308645.1
Coordinates 716867..717352 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LVQ70_RS03545 Protein ID WP_004150912.1
Coordinates 716577..716870 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ70_RS03525 (LVQ70_03525) 711787..712389 - 603 WP_025367887.1 short chain dehydrogenase -
LVQ70_RS03530 (LVQ70_03530) 712487..713398 + 912 WP_021314148.1 LysR family transcriptional regulator -
LVQ70_RS03535 (LVQ70_03535) 713399..714547 - 1149 WP_020316731.1 PLP-dependent aspartate aminotransferase family protein -
LVQ70_RS03540 (LVQ70_03540) 714558..715934 - 1377 WP_004181304.1 cystathionine beta-synthase -
LVQ70_RS03545 (LVQ70_03545) 716577..716870 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LVQ70_RS03550 (LVQ70_03550) 716867..717352 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
LVQ70_RS03555 (LVQ70_03555) 718056..718650 + 595 Protein_697 hypothetical protein -
LVQ70_RS03560 (LVQ70_03560) 718747..718963 + 217 Protein_698 transposase -
LVQ70_RS03570 (LVQ70_03570) 719641..720354 - 714 WP_025367888.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 718747..718899 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T229533 WP_009308645.1 NZ_CP089877:716867-717352 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T229533 NZ_CP124488:60672-61052 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT229533 WP_004150912.1 NZ_CP089877:716577-716870 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT229533 NZ_CP124488:60451-60672 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References