Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2322222..2322997 Replicon chromosome
Accession NZ_CP063922
Organism Klebsiella pneumoniae strain BM338-1

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag ISA71_RS11405 Protein ID WP_004889762.1
Coordinates 2322222..2322707 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ISA71_RS11410 Protein ID WP_004150912.1
Coordinates 2322704..2322997 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA71_RS11385 2319385..2320098 + 714 WP_002916694.1 DUF554 domain-containing protein -
ISA71_RS11395 2320613..2320829 - 217 Protein_2235 transposase -
ISA71_RS11400 2320926..2321518 - 593 Protein_2236 hypothetical protein -
ISA71_RS11405 2322222..2322707 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
ISA71_RS11410 2322704..2322997 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ISA71_RS11415 2323641..2325017 + 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
ISA71_RS11420 2325028..2326176 + 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
ISA71_RS11425 2326177..2327088 - 912 WP_017879796.1 LysR family transcriptional regulator -
ISA71_RS11430 2327186..2327788 + 603 WP_004174410.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2320677..2320829 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17553.50 Da        Isoelectric Point: 8.5144

>T179550 WP_004889762.1 NZ_CP063922:c2322707-2322222 [Klebsiella pneumoniae]
VISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T179550 NZ_CP084825:c5224357-5223974 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179550 WP_004150912.1 NZ_CP063922:c2322997-2322704 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179550 NZ_CP084825:c5224599-5224357 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAGGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References