Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743542..744317 Replicon chromosome
Accession NZ_CP090343
Organism Klebsiella pneumoniae strain KPK3

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LXL84_RS03710 Protein ID WP_004150910.1
Coordinates 743832..744317 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LXL84_RS03705 Protein ID WP_004150912.1
Coordinates 743542..743835 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXL84_RS03685 (LXL84_03685) 738750..739352 - 603 WP_062954968.1 short chain dehydrogenase -
LXL84_RS03690 (LXL84_03690) 739450..740361 + 912 WP_004188550.1 LysR family transcriptional regulator -
LXL84_RS03695 (LXL84_03695) 740362..741510 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LXL84_RS03700 (LXL84_03700) 741521..742897 - 1377 WP_004217775.1 cystathionine beta-synthase -
LXL84_RS03705 (LXL84_03705) 743542..743835 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LXL84_RS03710 (LXL84_03710) 743832..744317 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LXL84_RS03715 (LXL84_03715) 745021..745614 + 594 WP_004188553.1 hypothetical protein -
LXL84_RS03720 (LXL84_03720) 745711..745927 + 217 Protein_732 transposase -
LXL84_RS03725 (LXL84_03725) 746533..747405 + 873 WP_004188557.1 ParA family protein -
LXL84_RS03730 (LXL84_03730) 747405..747788 + 384 WP_004150906.1 hypothetical protein -
LXL84_RS03735 (LXL84_03735) 747781..749148 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 745711..745863 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T230440 WP_004150910.1 NZ_CP090343:743832-744317 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T230440 NZ_CP124899:66479-66589 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAACCGAAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT230440 WP_004150912.1 NZ_CP090343:743542-743835 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT230440 NZ_CP124899:c66693-66629 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References