Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739923..740698 Replicon chromosome
Accession NZ_CP061385
Organism Klebsiella pneumoniae strain NU-CRE213

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HCV85_RS03685 Protein ID WP_004150910.1
Coordinates 740213..740698 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HCV85_RS03680 Protein ID WP_004150912.1
Coordinates 739923..740216 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV85_RS03660 735131..735733 - 603 WP_002916735.1 short chain dehydrogenase -
HCV85_RS03665 735831..736742 + 912 WP_004188550.1 LysR family transcriptional regulator -
HCV85_RS03670 736743..737891 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HCV85_RS03675 737902..739278 - 1377 WP_004217775.1 cystathionine beta-synthase -
HCV85_RS03680 739923..740216 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HCV85_RS03685 740213..740698 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HCV85_RS03690 741402..741995 + 594 WP_004188553.1 hypothetical protein -
HCV85_RS03695 742092..742308 + 217 Protein_727 transposase -
HCV85_RS03700 742914..743786 + 873 WP_004188557.1 ParA family protein -
HCV85_RS03705 743786..744169 + 384 WP_116962211.1 hypothetical protein -
HCV85_RS03710 744162..745529 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742092..742244 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T172554 WP_004150910.1 NZ_CP061385:740213-740698 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T172554 NZ_CP079780:c3918489-3918211 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT172554 WP_004150912.1 NZ_CP061385:739923-740216 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT172554 NZ_CP079780:c3918752-3918492 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References