Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737822..738597 Replicon chromosome
Accession NZ_CP006923
Organism Klebsiella pneumoniae 30660/NJST258_1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KPNJ1_RS03745 Protein ID WP_004150910.1
Coordinates 738112..738597 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPNJ1_RS03740 Protein ID WP_004150912.1
Coordinates 737822..738115 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ1_RS03720 733030..733632 - 603 WP_002916735.1 short chain dehydrogenase -
KPNJ1_RS03725 733730..734641 + 912 WP_004188550.1 LysR family transcriptional regulator -
KPNJ1_RS03730 734642..735790 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KPNJ1_RS03735 735801..737177 - 1377 WP_004217775.1 cystathionine beta-synthase -
KPNJ1_RS03740 737822..738115 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPNJ1_RS03745 738112..738597 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KPNJ1_RS03750 739301..739894 + 594 WP_004188553.1 hypothetical protein -
KPNJ1_RS03755 739991..740207 + 217 Protein_725 transposase -
KPNJ1_RS03760 740813..741685 + 873 WP_004188557.1 ParA family protein -
KPNJ1_RS03765 741685..742068 + 384 WP_004150906.1 hypothetical protein -
KPNJ1_RS03770 742061..743428 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739991..740143 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T45751 WP_004150910.1 NZ_CP006923:738112-738597 [Klebsiella pneumoniae 30660/NJST258_1]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T45751 NZ_CP006923:738112-738597 [Klebsiella pneumoniae 30660/NJST258_1]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT45751 WP_004150912.1 NZ_CP006923:737822-738115 [Klebsiella pneumoniae 30660/NJST258_1]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT45751 NZ_CP006923:737822-738115 [Klebsiella pneumoniae 30660/NJST258_1]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References