Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 775408..776183 Replicon chromosome
Accession NZ_CP040833
Organism Klebsiella pneumoniae strain SH12

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag FHY43_RS03920 Protein ID WP_004150910.1
Coordinates 775698..776183 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FHY43_RS03915 Protein ID WP_004150912.1
Coordinates 775408..775701 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHY43_RS03895 (FHY43_03920) 770616..771218 - 603 WP_062954968.1 short chain dehydrogenase -
FHY43_RS03900 (FHY43_03925) 771316..772227 + 912 WP_004188550.1 LysR family transcriptional regulator -
FHY43_RS03905 (FHY43_03930) 772228..773376 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
FHY43_RS03910 (FHY43_03935) 773387..774763 - 1377 WP_004217775.1 cystathionine beta-synthase -
FHY43_RS03915 (FHY43_03940) 775408..775701 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FHY43_RS03920 (FHY43_03945) 775698..776183 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
FHY43_RS03925 (FHY43_03950) 776887..777480 + 594 WP_004188553.1 hypothetical protein -
FHY43_RS03930 (FHY43_03955) 777577..777793 + 217 Protein_774 transposase -
FHY43_RS03935 (FHY43_03970) 778399..779271 + 873 WP_004188557.1 ParA family protein -
FHY43_RS03940 (FHY43_03975) 779271..779654 + 384 WP_004150906.1 hypothetical protein -
FHY43_RS03945 (FHY43_03980) 779647..781014 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 777577..777729 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T127066 WP_004150910.1 NZ_CP040833:775698-776183 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T127066 NZ_CP040833:775698-776183 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT127066 WP_004150912.1 NZ_CP040833:775408-775701 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT127066 NZ_CP040833:775408-775701 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References