Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737218..737993 Replicon chromosome
Accession NZ_CP089014
Organism Klebsiella pneumoniae strain 53374

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LRZ80_RS03690 Protein ID WP_004150910.1
Coordinates 737508..737993 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LRZ80_RS03685 Protein ID WP_004150912.1
Coordinates 737218..737511 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ80_RS03665 (LRZ80_03665) 732425..733027 - 603 WP_002916735.1 short chain dehydrogenase -
LRZ80_RS03670 (LRZ80_03670) 733125..734037 + 913 Protein_721 LysR family transcriptional regulator -
LRZ80_RS03675 (LRZ80_03675) 734038..735186 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LRZ80_RS03680 (LRZ80_03680) 735197..736573 - 1377 WP_004217775.1 cystathionine beta-synthase -
LRZ80_RS03685 (LRZ80_03685) 737218..737511 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LRZ80_RS03690 (LRZ80_03690) 737508..737993 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LRZ80_RS03695 (LRZ80_03695) 738697..739290 + 594 WP_004188553.1 hypothetical protein -
LRZ80_RS03700 (LRZ80_03700) 739387..739603 + 217 Protein_727 transposase -
LRZ80_RS03705 (LRZ80_03705) 740209..741081 + 873 WP_004188557.1 ParA family protein -
LRZ80_RS03710 (LRZ80_03710) 741081..741464 + 384 WP_004150906.1 hypothetical protein -
LRZ80_RS03715 (LRZ80_03715) 741457..742824 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739387..739539 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T227364 WP_004150910.1 NZ_CP089014:737508-737993 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T227364 NZ_CP122848:c39167-38787 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGATATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT227364 WP_004150912.1 NZ_CP089014:737218-737511 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT227364 NZ_CP122848:c39388-39167 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References