Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4536503..4537278 Replicon chromosome
Accession NZ_CP008831
Organism Klebsiella pneumoniae subsp. pneumoniae KPR0928

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KPR0928_RS22855 Protein ID WP_004150910.1
Coordinates 4536503..4536988 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPR0928_RS22860 Protein ID WP_004150912.1
Coordinates 4536985..4537278 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPR0928_RS22830 4531672..4533039 - 1368 WP_004150905.1 replicative DNA helicase -
KPR0928_RS22835 4533032..4533415 - 384 WP_004150906.1 hypothetical protein -
KPR0928_RS22840 4533415..4534287 - 873 WP_004188557.1 ParA family protein -
KPR0928_RS22845 4534893..4535109 - 217 Protein_4355 transposase -
KPR0928_RS22850 4535206..4535799 - 594 WP_004188553.1 hypothetical protein -
KPR0928_RS22855 4536503..4536988 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KPR0928_RS22860 4536985..4537278 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPR0928_RS22865 4537923..4539299 + 1377 WP_004217775.1 cystathionine beta-synthase -
KPR0928_RS22870 4539310..4540458 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KPR0928_RS22875 4540459..4541370 - 912 WP_004188550.1 LysR family transcriptional regulator -
KPR0928_RS22880 4541468..4542070 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4479482..4537529 58047
flank IS/Tn - - 4534957..4535109 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T47680 WP_004150910.1 NZ_CP008831:c4536988-4536503 [Klebsiella pneumoniae subsp. pneumoniae KPR0928]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T47680 NZ_CP008831:c4536988-4536503 [Klebsiella pneumoniae subsp. pneumoniae KPR0928]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT47680 WP_004150912.1 NZ_CP008831:c4537278-4536985 [Klebsiella pneumoniae subsp. pneumoniae KPR0928]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT47680 NZ_CP008831:c4537278-4536985 [Klebsiella pneumoniae subsp. pneumoniae KPR0928]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References