Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1035908..1036683 Replicon chromosome
Accession NZ_CP018306
Organism Klebsiella pneumoniae strain Klebsiella pneumoniae 459 isolate 459

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag A9493_RS05190 Protein ID WP_004150910.1
Coordinates 1035908..1036393 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag A9493_RS05195 Protein ID WP_004150912.1
Coordinates 1036390..1036683 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9493_RS05160 1031076..1032443 - 1368 WP_004150905.1 replicative DNA helicase -
A9493_RS05165 1032436..1032819 - 384 WP_004150906.1 hypothetical protein -
A9493_RS05170 1032819..1033691 - 873 WP_004188557.1 ParA family protein -
A9493_RS05180 1034298..1034514 - 217 Protein_989 transposase -
A9493_RS05185 1034611..1035204 - 594 WP_004188553.1 hypothetical protein -
A9493_RS05190 1035908..1036393 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
A9493_RS05195 1036390..1036683 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
A9493_RS05200 1037328..1038704 + 1377 WP_004217775.1 cystathionine beta-synthase -
A9493_RS05205 1038715..1039863 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
A9493_RS05210 1039864..1040775 - 912 WP_004188550.1 LysR family transcriptional regulator -
A9493_RS05215 1040873..1041475 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 978886..1036934 58048
flank IS/Tn - - 1034362..1034514 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T69668 WP_004150910.1 NZ_CP018306:c1036393-1035908 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T69668 NZ_CP018306:c1036393-1035908 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT69668 WP_004150912.1 NZ_CP018306:c1036683-1036390 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT69668 NZ_CP018306:c1036683-1036390 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References