Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4883354..4884129 Replicon chromosome
Accession NZ_CP066534
Organism Klebsiella pneumoniae strain WCHKP090374

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag H3G96_RS24010 Protein ID WP_004150910.1
Coordinates 4883354..4883839 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag H3G96_RS24015 Protein ID WP_004150912.1
Coordinates 4883836..4884129 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G96_RS23985 4878523..4879890 - 1368 WP_004150905.1 replicative DNA helicase -
H3G96_RS23990 4879883..4880266 - 384 WP_004150906.1 hypothetical protein -
H3G96_RS23995 4880266..4881138 - 873 WP_004188557.1 ParA family protein -
H3G96_RS24000 4881744..4881960 - 217 Protein_4711 transposase -
H3G96_RS24005 4882057..4882650 - 594 WP_004188553.1 hypothetical protein -
H3G96_RS24010 4883354..4883839 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
H3G96_RS24015 4883836..4884129 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
H3G96_RS24020 4884475..4885172 + 698 Protein_4715 IS1 family transposase -
H3G96_RS24025 4885551..4886927 + 1377 WP_004217775.1 cystathionine beta-synthase -
H3G96_RS24030 4886938..4888086 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
H3G96_RS24035 4888087..4888998 - 912 WP_004188550.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4825134..4884380 59246
- inside IScluster/Tn - - 4881808..4885172 3364


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T184605 WP_004150910.1 NZ_CP066534:c4883839-4883354 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T184605 NZ_CP087694:2312977-2313255 [Xylella taiwanensis]
ATGATTAAAACCTTTGCCGACCAGGACACGGAATCTTTGTTCAACGGGTCACGGGTACGTCGCTTTATTCACATAGAAGC
GGTCGCACGCCGGAAGCTGATTCAATTGCATGCAGCAGCGACCTTGAGCTTTCTCAATGCGCCGCCTGGTAATCGGCTTG
AAACCTTAAAAGGGGACCGCCAAGGGCAGCACAGCATCCGTATCAACAATCAATTCCGCCTCTGTTTCATTTGGGAAAAC
GGCGATGCCTATCAGGTTGAAATTGTCGATTATCACTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT184605 WP_004150912.1 NZ_CP066534:c4884129-4883836 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT184605 NZ_CP087694:2313259-2313558 [Xylella taiwanensis]
ATGAGCATGTCCAAGACACTTCCCCCCATTCACCCAGGAGAAATTCTCCGGGAAGAATTTATGGCCCCTCTAGGCTTATC
CAGCAATGCGCTGGCCAAGGCGATTGGCGTGACGGCGGCACGGATCAACGAGATTGTGCGCGAACGACGTGGGATCACGG
CGGATACTGCACTGCGTCTGGCACGTTACTTTGGCACCGATCCGCAAAGCTGGCTGAATCTGGAACAGCACTATGCACTC
GAATGCGCCAAACGCGACCTTGGAGAGGCCCTGTCACGTATTCACCCTCGCGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References