Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736483..737258 Replicon chromosome
Accession NZ_CP054170
Organism Klebsiella pneumoniae strain BA12818

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HBJ12_RS03675 Protein ID WP_004150910.1
Coordinates 736773..737258 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HBJ12_RS03670 Protein ID WP_004150912.1
Coordinates 736483..736776 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ12_RS03650 731691..732293 - 603 WP_002916735.1 short chain dehydrogenase -
HBJ12_RS03655 732391..733302 + 912 WP_004188550.1 LysR family transcriptional regulator -
HBJ12_RS03660 733303..734451 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HBJ12_RS03665 734462..735838 - 1377 WP_004217775.1 cystathionine beta-synthase -
HBJ12_RS03670 736483..736776 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HBJ12_RS03675 736773..737258 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HBJ12_RS03680 737962..738555 + 594 WP_004188553.1 hypothetical protein -
HBJ12_RS03685 738652..738868 + 217 Protein_724 transposase -
HBJ12_RS03690 739474..740346 + 873 WP_166701498.1 ParA family protein -
HBJ12_RS03695 740346..740729 + 384 WP_004150906.1 hypothetical protein -
HBJ12_RS03700 740722..742089 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 738652..738804 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T158887 WP_004150910.1 NZ_CP054170:736773-737258 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T158887 NZ_CP069882:176976-177353 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT158887 WP_004150912.1 NZ_CP054170:736483-736776 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT158887 NZ_CP069882:176519-176899 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCGCCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References