Detailed information of TA system TA226062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 756066..756841
• Replicon: chromosome
• Accession: NZ_CP087706
• Organism: Klebsiella pneumoniae strain YZH2487

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LO768_RS03790
• Protein ID: WP_114263821.1
• Coordinates: 756356..756841 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: LO768_RS03785
• Protein ID: WP_004150912.1
• Coordinates: 756066..756359 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO768_RS03765 (751274)	751274..751876	-	603	WP_004174410.1	short chain dehydrogenase	-
LO768_RS03770 (751974)	751974..752885	+	912	WP_023342207.1	LysR family transcriptional regulator	-
LO768_RS03775 (752886)	752886..754034	-	1149	WP_114263822.1	PLP-dependent aspartate aminotransferase family protein	-
LO768_RS03780 (754045)	754045..755421	-	1377	WP_004181304.1	cystathionine beta-synthase	-
LO768_RS03785 (756066)	756066..756359	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
LO768_RS03790 (756356)	756356..756841	+	486	WP_114263821.1	GNAT family N-acetyltransferase	Toxin
LO768_RS03795 (757545)	757545..758138	+	594	WP_004188553.1	hypothetical protein	-
LO768_RS03800 (758235)	758235..758451	+	217	Protein_746	transposase	-
LO768_RS03810 (759129)	759129..759842	-	714	WP_002916694.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_016845 Klebsiella pneumoniae subsp. pneumoniae HS11286 IS/Tn TA026030 4559372..4559524 0	flank	IS/Tn	-	-	758235..758387	152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17611.60 Da
• Isoelectric Point: 8.5144

>T226062 WP_114263821.1 NZ_CP087706:756356-756841 [Klebsiella pneumoniae]
MISAPEPLHSGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Nucleotide

• Length: 486 bp

>T226062 NZ_CP121768:c482718-482380 [Glaesserella parasuis]
ATGAATGTCGCTTTTGTGGAGTTGCCGCCTTTTGAGGAATATAGAAAAAAGTATCTTGACGATGACACATTTAGATTATT
ACAAAATGAGTTATTAAAATTTCCCGATAAGGGAGAGCTTATTCAAGGTACTGGGGGACTAAGAAAATTGCGTATTGTGG
ATATTATTAGACAAAAAGGAAAAAGAGGCGGAGCTAGGGTAATCTATTACTATTATGTACAAGGGAAACAAGTTTGGTTG
TTCCATGCCTATAATAAAAACCAACAAGACGATTTATCTAATGAAGAACGTGTGGTTTTGGCGAATACATTAAGCTATTT
AAAATCTTTAGTGAGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT226062 WP_004150912.1 NZ_CP087706:756066-756359 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT226062 NZ_CP121768:c482376-482068 [Glaesserella parasuis]
ATGGGAAAATTATTTAATGAACTAATGGAAGGGATGCAAGCATTAGAGTTACACTTACAAGGCAAAATTACACTAAAAAC
CACTGTTGTGGAAGTTCCTGATCCGATAGTGATTACCCCTGAAGAAGTGAAAGCTATTCGCCAAAAATTAAATCTTTCAC
AAGCGGTTTTTGCTCGTAAATTACACACTTCGGTGAGAACTTATCAAGGTTGGGAGCAAGGAAGAACAAAGCCCAATCCG
CAAGCGGTCTTACTATTAAAAATGGTTGAAAAATCGCCAAAAACATTTGAGCAAATTGCGAGCATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4