Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737546..738321 Replicon chromosome
Accession NZ_CP115930
Organism Klebsiella pneumoniae subsp. pneumoniae strain 43UC01

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag DLD89_RS03685 Protein ID WP_004150910.1
Coordinates 737836..738321 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag DLD89_RS03680 Protein ID WP_004150912.1
Coordinates 737546..737839 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DLD89_RS03660 (DLD89_03660) 732754..733356 - 603 WP_002916735.1 short chain dehydrogenase -
DLD89_RS03665 (DLD89_03665) 733454..734365 + 912 WP_004188550.1 LysR family transcriptional regulator -
DLD89_RS03670 (DLD89_03670) 734366..735514 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
DLD89_RS03675 (DLD89_03675) 735525..736901 - 1377 WP_004217775.1 cystathionine beta-synthase -
DLD89_RS03680 (DLD89_03680) 737546..737839 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
DLD89_RS03685 (DLD89_03685) 737836..738321 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
DLD89_RS03690 (DLD89_03690) 739025..739618 + 594 WP_004188553.1 hypothetical protein -
DLD89_RS03695 (DLD89_03695) 739715..739931 + 217 Protein_726 transposase -
DLD89_RS03700 (DLD89_03700) 740537..741409 + 873 WP_004188557.1 ParA family protein -
DLD89_RS03705 (DLD89_03705) 741409..741792 + 384 WP_004150906.1 hypothetical protein -
DLD89_RS03710 (DLD89_03710) 741785..743152 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739715..739867 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T267499 WP_004150910.1 NZ_CP115930:737836-738321 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT267499 WP_004150912.1 NZ_CP115930:737546-737839 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References