Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5002493..5003268 Replicon chromosome
Accession NZ_CP084870
Organism Klebsiella pneumoniae strain BJ1

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag LGM01_RS23810 Protein ID WP_032445441.1
Coordinates 5002493..5002978 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LGM01_RS23815 Protein ID WP_004150912.1
Coordinates 5002975..5003268 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM01_RS23790 (4999329) 4999329..5000042 + 714 WP_002916694.1 DUF554 domain-containing protein -
LGM01_RS23800 (5000883) 5000883..5001099 - 217 Protein_4639 transposase -
LGM01_RS23805 (5001196) 5001196..5001789 - 594 WP_032445442.1 hypothetical protein -
LGM01_RS23810 (5002493) 5002493..5002978 - 486 WP_032445441.1 GNAT family N-acetyltransferase Toxin
LGM01_RS23815 (5002975) 5002975..5003268 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LGM01_RS23820 (5003913) 5003913..5005289 + 1377 WP_072158501.1 cystathionine beta-synthase -
LGM01_RS23825 (5005300) 5005300..5006448 + 1149 WP_016947441.1 PLP-dependent aspartate aminotransferase family protein -
LGM01_RS23830 (5006449) 5006449..5007360 - 912 WP_004181308.1 LysR family transcriptional regulator -
LGM01_RS23835 (5007458) 5007458..5008060 + 603 WP_020802400.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 5000947..5001099 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17580.59 Da        Isoelectric Point: 8.1558

>T219691 WP_032445441.1 NZ_CP084870:c5002978-5002493 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGIGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T219691 NZ_CP116579:c2336695-2336345 [Enterococcus durans]
ATGGTGAGAAACCCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACATGAACAAACAGGAAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCAGATTATAGTAACGTTGTTACAGTTGCTCCGATTTCTAGTACAACTA
GAAACTATCCGTTATATGTAAATATTAATTCAAAATATGAAATGAAGACAACCGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCACATGATAGTTTGGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTATTTCAAAAAGTAAGTAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT219691 WP_004150912.1 NZ_CP084870:c5003268-5002975 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT219691 NZ_CP116579:c2336952-2336689 [Enterococcus durans]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGTCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAACACAAGAAGAAGTAAACGGTCAAAAGCGGCTAATCATCGAAGCGGCTAATTTAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGACGAAAGAAACCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References