Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 769272..770047 Replicon chromosome
Accession NZ_CP089097
Organism Klebsiella pneumoniae strain EH13

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag LSG42_RS03870 Protein ID WP_009308645.1
Coordinates 769562..770047 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LSG42_RS03865 Protein ID WP_004150912.1
Coordinates 769272..769565 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSG42_RS03845 (LSG42_03845) 764480..765082 - 603 WP_004174410.1 short chain dehydrogenase -
LSG42_RS03850 (LSG42_03850) 765180..766091 + 912 WP_023288001.1 LysR family transcriptional regulator -
LSG42_RS03855 (LSG42_03855) 766092..767240 - 1149 WP_032437260.1 PLP-dependent aspartate aminotransferase family protein -
LSG42_RS03860 (LSG42_03860) 767251..768627 - 1377 WP_074187679.1 pyridoxal-phosphate dependent enzyme -
LSG42_RS03865 (LSG42_03865) 769272..769565 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LSG42_RS03870 (LSG42_03870) 769562..770047 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
LSG42_RS03875 (LSG42_03875) 770751..771344 + 594 WP_004188553.1 hypothetical protein -
LSG42_RS03880 (LSG42_03880) 771441..771657 + 217 Protein_764 transposase -
LSG42_RS03890 (LSG42_03890) 772172..772885 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 771441..771593 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T227631 WP_009308645.1 NZ_CP089097:769562-770047 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T227631 NZ_CP123008:1569092-1569310 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT227631 WP_004150912.1 NZ_CP089097:769272-769565 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT227631 NZ_CP123008:1568692-1569066 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References