Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5369936..5370711 Replicon chromosome
Accession NZ_CP023722
Organism Klebsiella pneumoniae strain TVGHCRE225

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CO233_RS27270 Protein ID WP_004150910.1
Coordinates 5369936..5370421 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CO233_RS27275 Protein ID WP_004150912.1
Coordinates 5370418..5370711 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO233_RS27240 5365105..5366472 - 1368 WP_004150905.1 replicative DNA helicase -
CO233_RS27245 5366465..5366848 - 384 WP_004150906.1 hypothetical protein -
CO233_RS27250 5366848..5367720 - 873 WP_004188557.1 ParA family protein -
CO233_RS27260 5368326..5368542 - 217 Protein_5150 transposase -
CO233_RS27265 5368639..5369232 - 594 WP_004188553.1 hypothetical protein -
CO233_RS27270 5369936..5370421 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CO233_RS27275 5370418..5370711 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CO233_RS27280 5371356..5372732 + 1377 WP_004217775.1 cystathionine beta-synthase -
CO233_RS27285 5372743..5373891 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CO233_RS27290 5373892..5374803 - 912 WP_004188550.1 LysR family transcriptional regulator -
CO233_RS27295 5374901..5375503 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 5312916..5370962 58046
flank IS/Tn - - 5368390..5368542 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T85958 WP_004150910.1 NZ_CP023722:c5370421-5369936 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T85958 NZ_CP023722:c5370421-5369936 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT85958 WP_004150912.1 NZ_CP023722:c5370711-5370418 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT85958 NZ_CP023722:c5370711-5370418 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References