Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741262..742037 Replicon chromosome
Accession NZ_AP024772
Organism Klebsiella pneumoniae strain TUM12133

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6J98_RS03710 Protein ID WP_004150910.1
Coordinates 741552..742037 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6J98_RS03705 Protein ID WP_004150912.1
Coordinates 741262..741555 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J98_RS03685 (TUM12133_07210) 736470..737072 - 603 WP_002916735.1 short chain dehydrogenase -
K6J98_RS03690 (TUM12133_07220) 737170..738081 + 912 WP_004188550.1 LysR family transcriptional regulator -
K6J98_RS03695 (TUM12133_07230) 738082..739230 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6J98_RS03700 (TUM12133_07240) 739241..740617 - 1377 WP_004217775.1 cystathionine beta-synthase -
K6J98_RS03705 (TUM12133_07250) 741262..741555 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6J98_RS03710 (TUM12133_07260) 741552..742037 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6J98_RS03715 (TUM12133_07280) 742741..743334 + 594 WP_004188553.1 hypothetical protein -
K6J98_RS03720 (TUM12133_07290) 743431..743647 + 217 Protein_732 transposase -
K6J98_RS03725 (TUM12133_07300) 744253..745125 + 873 WP_004188557.1 ParA family protein -
K6J98_RS03730 (TUM12133_07310) 745125..745508 + 384 WP_004150906.1 hypothetical protein -
K6J98_RS03735 (TUM12133_07320) 745501..746868 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743431..743583 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T39562 WP_004150910.1 NZ_AP024772:741552-742037 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T39562 NZ_AP024772:741552-742037 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT39562 WP_004150912.1 NZ_AP024772:741262-741555 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT39562 NZ_AP024772:741262-741555 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References