Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3890858..3891633 Replicon chromosome
Accession NZ_CP065441
Organism Klebsiella pneumoniae strain Patrick Star

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I5M63_RS19215 Protein ID WP_004150910.1
Coordinates 3891148..3891633 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I5M63_RS19210 Protein ID WP_004150912.1
Coordinates 3890858..3891151 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M63_RS19190 3886066..3886668 - 603 WP_062954968.1 short chain dehydrogenase -
I5M63_RS19195 3886766..3887677 + 912 WP_004188550.1 LysR family transcriptional regulator -
I5M63_RS19200 3887678..3888826 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I5M63_RS19205 3888837..3890213 - 1377 WP_004217775.1 cystathionine beta-synthase -
I5M63_RS19210 3890858..3891151 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I5M63_RS19215 3891148..3891633 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I5M63_RS19220 3892337..3892930 + 594 WP_004188553.1 hypothetical protein -
I5M63_RS19225 3893027..3893243 + 217 Protein_3761 transposase -
I5M63_RS19230 3893849..3894721 + 873 WP_004188557.1 ParA family protein -
I5M63_RS19235 3894721..3895104 + 384 WP_004150906.1 hypothetical protein -
I5M63_RS19240 3895097..3896464 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3893027..3893179 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T182668 WP_004150910.1 NZ_CP065441:3891148-3891633 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182668 NZ_CP086532:1042992-1043327 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182668 WP_004150912.1 NZ_CP065441:3890858-3891151 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182668 NZ_CP086532:1042744-1042992 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References