194408

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4545365..4546140 Replicon chromosome
Accession NZ_CP070579
Organism Klebsiella pneumoniae strain KP52810

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JW303_RS24185 Protein ID WP_004150910.1
Coordinates 4545365..4545850 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JW303_RS24190 Protein ID WP_004150912.1
Coordinates 4545847..4546140 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW303_RS24160 4540534..4541901 - 1368 WP_004150905.1 replicative DNA helicase -
JW303_RS24165 4541894..4542277 - 384 WP_004150906.1 hypothetical protein -
JW303_RS24170 4542277..4543149 - 873 WP_004188557.1 ParA family protein -
JW303_RS24175 4543755..4543971 - 217 Protein_4317 transposase -
JW303_RS24180 4544068..4544661 - 594 WP_004188553.1 hypothetical protein -
JW303_RS24185 4545365..4545850 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JW303_RS24190 4545847..4546140 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JW303_RS24195 4546785..4548161 + 1377 WP_004217775.1 cystathionine beta-synthase -
JW303_RS24200 4548172..4549320 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
JW303_RS24205 4549321..4550232 - 912 WP_004188550.1 LysR family transcriptional regulator -
JW303_RS24210 4550330..4550932 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4488344..4546391 58047
flank IS/Tn - - 4543819..4543971 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T194408 WP_004150910.1 NZ_CP070579:c4545850-4545365 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T194408 NZ_CP093472:c85581-85099 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT194408 WP_004150912.1 NZ_CP070579:c4546140-4545847 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT194408 NZ_CP093472:c85835-85569 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References