Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5030693..5031468 Replicon chromosome
Accession NZ_CP125086
Organism Klebsiella pneumoniae subsp. pneumoniae strain HVKP2

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag QFB74_RS25425 Protein ID WP_004889762.1
Coordinates 5030983..5031468 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag QFB74_RS25420 Protein ID WP_004150912.1
Coordinates 5030693..5030986 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QFB74_RS25400 (5025902) 5025902..5026504 - 603 WP_002916735.1 short chain dehydrogenase -
QFB74_RS25405 (5026602) 5026602..5027513 + 912 WP_014906929.1 LysR family transcriptional regulator -
QFB74_RS25410 (5027514) 5027514..5028662 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
QFB74_RS25415 (5028673) 5028673..5030049 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
QFB74_RS25420 (5030693) 5030693..5030986 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
QFB74_RS25425 (5030983) 5030983..5031468 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
QFB74_RS25430 (5032172) 5032172..5032765 + 594 WP_004188553.1 hypothetical protein -
QFB74_RS25435 (5032862) 5032862..5033078 + 217 Protein_4733 transposase -
QFB74_RS25445 (5033756) 5033756..5034469 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 5032862..5033014 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T281897 WP_004889762.1 NZ_CP125086:5030983-5031468 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT281897 WP_004150912.1 NZ_CP125086:5030693-5030986 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References