Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2160054..2160829 Replicon chromosome
Accession NZ_CP017994
Organism Klebsiella pneumoniae strain P1428

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag BMF28_RS10740 Protein ID WP_004889762.1
Coordinates 2160054..2160539 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag BMF28_RS10745 Protein ID WP_004150912.1
Coordinates 2160536..2160829 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BMF28_RS10715 2157053..2157766 + 714 WP_002916694.1 DUF554 domain-containing protein -
BMF28_RS10730 2158444..2158660 - 217 Protein_2036 transposase -
BMF28_RS10735 2158757..2159350 - 594 WP_004188553.1 hypothetical protein -
BMF28_RS10740 2160054..2160539 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
BMF28_RS10745 2160536..2160829 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
BMF28_RS10750 2161473..2162849 + 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
BMF28_RS10755 2162860..2164008 + 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
BMF28_RS10760 2164009..2164920 - 912 WP_014906929.1 LysR family transcriptional regulator -
BMF28_RS10765 2165018..2165620 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2158508..2158660 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T68507 WP_004889762.1 NZ_CP017994:c2160539-2160054 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T68507 NZ_CP017994:c2160539-2160054 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT68507 WP_004150912.1 NZ_CP017994:c2160829-2160536 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT68507 NZ_CP017994:c2160829-2160536 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References