Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1546483..1547258 Replicon chromosome
Accession NZ_CP084168
Organism Klebsiella pneumoniae strain B17602

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag LDH50_RS07435 Protein ID WP_004889762.1
Coordinates 1546483..1546968 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LDH50_RS07440 Protein ID WP_004150912.1
Coordinates 1546965..1547258 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDH50_RS07415 (LDH50_07415) 1543646..1544359 + 714 WP_002916694.1 DUF554 domain-containing protein -
LDH50_RS07425 (LDH50_07425) 1544874..1545090 - 217 Protein_1441 transposase -
LDH50_RS07430 (LDH50_07430) 1545187..1545779 - 593 Protein_1442 hypothetical protein -
LDH50_RS07435 (LDH50_07435) 1546483..1546968 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
LDH50_RS07440 (LDH50_07440) 1546965..1547258 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LDH50_RS07445 (LDH50_07445) 1547902..1549278 + 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
LDH50_RS07450 (LDH50_07450) 1549289..1550437 + 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
LDH50_RS07455 (LDH50_07455) 1550438..1551349 - 912 WP_017879796.1 LysR family transcriptional regulator -
LDH50_RS07460 (LDH50_07460) 1551447..1552049 + 603 WP_004174410.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1544938..1545090 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T218143 WP_004889762.1 NZ_CP084168:c1546968-1546483 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T218143 NZ_CP115654:c887690-887271 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT218143 WP_004150912.1 NZ_CP084168:c1547258-1546965 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT218143 NZ_CP115654:c887926-887690 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References