Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4559890..4560665 Replicon chromosome
Accession NZ_CP100511
Organism Klebsiella pneumoniae strain LH50-a

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag NLZ12_RS22280 Protein ID WP_021314147.1
Coordinates 4559890..4560375 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NLZ12_RS22285 Protein ID WP_004150912.1
Coordinates 4560372..4560665 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ12_RS22260 (NLZ12_22260) 4557052..4557765 + 714 WP_002916694.1 DUF554 domain-containing protein -
NLZ12_RS22270 (NLZ12_22270) 4558280..4558496 - 217 Protein_4371 transposase -
NLZ12_RS22275 (NLZ12_22275) 4558593..4559186 - 594 WP_004188553.1 hypothetical protein -
NLZ12_RS22280 (NLZ12_22280) 4559890..4560375 - 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
NLZ12_RS22285 (NLZ12_22285) 4560372..4560665 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NLZ12_RS22290 (NLZ12_22290) 4561310..4562686 + 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
NLZ12_RS22295 (NLZ12_22295) 4562697..4563845 + 1149 WP_020316731.1 PLP-dependent aspartate aminotransferase family protein -
NLZ12_RS22300 (NLZ12_22300) 4563846..4564757 - 912 WP_021314148.1 LysR family transcriptional regulator -
NLZ12_RS22305 (NLZ12_22305) 4564855..4565457 + 603 WP_004174410.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4558344..4558496 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T250718 WP_021314147.1 NZ_CP100511:c4560375-4559890 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT250718 WP_004150912.1 NZ_CP100511:c4560665-4560372 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References