Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4569829..4570604 Replicon chromosome
Accession NZ_CP063856
Organism Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag IOD44_RS22000 Protein ID WP_019704987.1
Coordinates 4570119..4570604 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IOD44_RS21995 Protein ID WP_004150912.1
Coordinates 4569829..4570122 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD44_RS21975 4565038..4565640 - 603 WP_020802400.1 short chain dehydrogenase -
IOD44_RS21980 4565738..4566649 + 912 WP_004188550.1 LysR family transcriptional regulator -
IOD44_RS21985 4566650..4567798 - 1149 WP_040223681.1 PLP-dependent aspartate aminotransferase family protein -
IOD44_RS21990 4567809..4569185 - 1377 WP_004217775.1 cystathionine beta-synthase -
IOD44_RS21995 4569829..4570122 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IOD44_RS22000 4570119..4570604 + 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
IOD44_RS22005 4571308..4571901 + 594 WP_004188553.1 hypothetical protein -
IOD44_RS22010 4571998..4572214 + 217 Protein_4294 transposase -
IOD44_RS22020 4572890..4573603 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4571998..4572150 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T179002 WP_019704987.1 NZ_CP063856:4570119-4570604 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T179002 NZ_CP084493:214549-214833 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179002 WP_004150912.1 NZ_CP063856:4569829-4570122 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179002 NZ_CP084493:214311-214559 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References