Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 727408..728183 Replicon chromosome
Accession NZ_CP067372
Organism Klebsiella pneumoniae strain BA30371

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag JI970_RS03580 Protein ID WP_021314147.1
Coordinates 727698..728183 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JI970_RS03575 Protein ID WP_004150912.1
Coordinates 727408..727701 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI970_RS03555 (722616) 722616..723218 - 603 WP_020802400.1 short chain dehydrogenase -
JI970_RS03560 (723316) 723316..724227 + 912 WP_004188550.1 LysR family transcriptional regulator -
JI970_RS03565 (724228) 724228..725376 - 1149 WP_040223681.1 PLP-dependent aspartate aminotransferase family protein -
JI970_RS03570 (725387) 725387..726763 - 1377 WP_074187260.1 cystathionine beta-synthase -
JI970_RS03575 (727408) 727408..727701 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JI970_RS03580 (727698) 727698..728183 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
JI970_RS03585 (728887) 728887..729480 + 594 WP_117259234.1 hypothetical protein -
JI970_RS03590 (729577) 729577..729793 + 217 Protein_705 transposase -
JI970_RS03600 (730634) 730634..731347 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 729577..729729 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T186198 WP_021314147.1 NZ_CP067372:727698-728183 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T186198 NZ_CP089270:c5196950-5196777 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT186198 WP_004150912.1 NZ_CP067372:727408-727701 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT186198 NZ_CP089270:c5197274-5196978 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References