Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741332..742107 Replicon chromosome
Accession NZ_CP065554
Organism Klebsiella pneumoniae strain FK 6768

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I7S93_RS03705 Protein ID WP_004150910.1
Coordinates 741622..742107 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I7S93_RS03700 Protein ID WP_004150912.1
Coordinates 741332..741625 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7S93_RS03680 (I7S93_03675) 736540..737142 - 603 WP_062954968.1 short chain dehydrogenase -
I7S93_RS03685 (I7S93_03680) 737240..738151 + 912 WP_004188550.1 LysR family transcriptional regulator -
I7S93_RS03690 (I7S93_03685) 738152..739300 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I7S93_RS03695 (I7S93_03690) 739311..740687 - 1377 WP_004217775.1 cystathionine beta-synthase -
I7S93_RS03700 (I7S93_03695) 741332..741625 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I7S93_RS03705 (I7S93_03700) 741622..742107 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I7S93_RS03710 (I7S93_03705) 742811..743404 + 594 WP_004188553.1 hypothetical protein -
I7S93_RS03715 (I7S93_03710) 743501..743717 + 217 Protein_730 transposase -
I7S93_RS03720 (I7S93_03715) 744323..745195 + 873 WP_004188557.1 ParA family protein -
I7S93_RS03725 (I7S93_03720) 745195..745578 + 384 WP_004150906.1 hypothetical protein -
I7S93_RS03730 (I7S93_03725) 745571..746938 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743501..743653 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T182904 WP_004150910.1 NZ_CP065554:741622-742107 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182904 NZ_CP086620:1303262-1303573 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182904 WP_004150912.1 NZ_CP065554:741332-741625 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182904 NZ_CP086620:1303570-1303989 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References