Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741317..742092 Replicon chromosome
Accession NZ_CP072996
Organism Klebsiella pneumoniae strain KP1878

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KA532_RS03700 Protein ID WP_004150910.1
Coordinates 741607..742092 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KA532_RS03695 Protein ID WP_004150912.1
Coordinates 741317..741610 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KA532_RS03675 (KA532_03675) 736525..737127 - 603 WP_062954968.1 short chain dehydrogenase -
KA532_RS03680 (KA532_03680) 737225..738136 + 912 WP_004188550.1 LysR family transcriptional regulator -
KA532_RS03685 (KA532_03685) 738137..739285 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KA532_RS03690 (KA532_03690) 739296..740672 - 1377 WP_004217775.1 cystathionine beta-synthase -
KA532_RS03695 (KA532_03695) 741317..741610 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KA532_RS03700 (KA532_03700) 741607..742092 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KA532_RS03705 (KA532_03705) 742796..743389 + 594 WP_004188553.1 hypothetical protein -
KA532_RS03710 (KA532_03710) 743486..743702 + 217 Protein_730 transposase -
KA532_RS03715 (KA532_03715) 744308..745180 + 873 WP_004188557.1 ParA family protein -
KA532_RS03720 (KA532_03720) 745180..745563 + 384 WP_004150906.1 hypothetical protein -
KA532_RS03725 (KA532_03725) 745556..746923 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743486..743638 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T199435 WP_004150910.1 NZ_CP072996:741607-742092 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T199435 NZ_CP097340:c941689-941420 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGCAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTATGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT199435 WP_004150912.1 NZ_CP072996:741317-741610 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT199435 NZ_CP097340:c941959-941693 [Mannheimia haemolytica]
ATGACGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References