Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2077010..2077785 Replicon chromosome
Accession NZ_CP044033
Organism Klebsiella pneumoniae strain FDAARGOS_631

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag FOB39_RS10435 Protein ID WP_009308645.1
Coordinates 2077300..2077785 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FOB39_RS10430 Protein ID WP_004150912.1
Coordinates 2077010..2077303 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB39_RS10410 2072218..2072820 - 603 WP_004174410.1 short chain dehydrogenase -
FOB39_RS10415 2072918..2073829 + 912 WP_004188550.1 LysR family transcriptional regulator -
FOB39_RS10420 2073830..2074978 - 1149 WP_065810255.1 PLP-dependent aspartate aminotransferase family protein -
FOB39_RS10425 2074989..2076365 - 1377 WP_072158501.1 cystathionine beta-synthase -
FOB39_RS10430 2077010..2077303 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FOB39_RS10435 2077300..2077785 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
FOB39_RS10440 2078489..2079082 + 594 WP_004188553.1 hypothetical protein -
FOB39_RS10445 2079179..2079395 + 217 Protein_1932 transposase -
FOB39_RS10460 2079910..2080623 - 714 WP_023284292.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2079179..2079331 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T139894 WP_009308645.1 NZ_CP044033:2077300-2077785 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T139894 NZ_CP058846:c3525724-3525317 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT139894 WP_004150912.1 NZ_CP044033:2077010-2077303 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT139894 NZ_CP058846:c3525971-3525705 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References