Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1879854..1880629 Replicon chromosome
Accession NZ_CP033756
Organism Klebsiella pneumoniae strain FDAARGOS_566

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag EGY03_RS10125 Protein ID WP_009308645.1
Coordinates 1879854..1880339 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag EGY03_RS10130 Protein ID WP_004150912.1
Coordinates 1880336..1880629 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY03_RS10095 1875022..1876389 - 1368 WP_040236167.1 replicative DNA helicase -
EGY03_RS10100 1876382..1876765 - 384 WP_040236166.1 hypothetical protein -
EGY03_RS10105 1876765..1877637 - 873 WP_040236165.1 ParA family protein -
EGY03_RS10115 1878244..1878460 - 217 Protein_1812 transposase -
EGY03_RS10120 1878557..1879150 - 594 WP_004188553.1 hypothetical protein -
EGY03_RS10125 1879854..1880339 - 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
EGY03_RS10130 1880336..1880629 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
EGY03_RS10135 1881274..1882650 + 1377 WP_072269297.1 cystathionine beta-synthase -
EGY03_RS10140 1882661..1883809 + 1149 WP_040236164.1 PLP-dependent aspartate aminotransferase family protein -
EGY03_RS10145 1883810..1884721 - 912 WP_004181308.1 LysR family transcriptional regulator -
EGY03_RS10150 1884819..1885421 + 603 WP_032448122.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1823158..1880880 57722
flank IS/Tn - - 1878308..1878460 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T114252 WP_009308645.1 NZ_CP033756:c1880339-1879854 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T114252 NZ_CP033756:c1880339-1879854 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT114252 WP_004150912.1 NZ_CP033756:c1880629-1880336 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT114252 NZ_CP033756:c1880629-1880336 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References