139146

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4464378..4465153 Replicon chromosome
Accession NZ_CP043597
Organism Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag B7D33_RS21945 Protein ID WP_019704987.1
Coordinates 4464378..4464863 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag B7D33_RS21950 Protein ID WP_004150912.1
Coordinates 4464860..4465153 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D33_RS21925 (4461057) 4461057..4461770 + 714 WP_002916694.1 DUF554 domain-containing protein -
B7D33_RS21935 (4462774) 4462774..4462990 - 217 Protein_4300 transposase -
B7D33_RS21940 (4463087) 4463087..4463680 - 594 Protein_4301 hypothetical protein -
B7D33_RS21945 (4464378) 4464378..4464863 - 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
B7D33_RS21950 (4464860) 4464860..4465153 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
B7D33_RS21955 (4465797) 4465797..4467173 + 1377 WP_004217775.1 cystathionine beta-synthase -
B7D33_RS21960 (4467184) 4467184..4468332 + 1149 WP_094645045.1 PLP-dependent aspartate aminotransferase family protein -
B7D33_RS21965 (4468333) 4468333..4469244 - 912 WP_004181308.1 LysR family transcriptional regulator -
B7D33_RS21970 (4469342) 4469342..4469944 + 603 WP_004174410.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4462838..4462990 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T139146 WP_019704987.1 NZ_CP043597:c4464863-4464378 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T139146 NZ_CP058410:c164966-164712 [Trichlorobacter lovleyi]
ATGATGATAAAATTCTCAACCGCAGCATGGGAGGACTATCTCTACTGGCAGACTACCGACAAGGCGATGCTGAAACGGAT
CAATCAACTTATCCGGGATATCCAGCGAGAACCGTTTGCCGGTATCGGCAAGCCGGAACCGCTCAAACATCAATTGGCCG
GTTTCTGGTCACGCAGAATAGACAGCTGCCACCGCTTGGTCTATGCGGTTGAAGGTGATACGCTCCTGATTGCACAGTGT
AGAGATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT139146 WP_004150912.1 NZ_CP043597:c4465153-4464860 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT139146 NZ_CP058410:c165214-164963 [Trichlorobacter lovleyi]
ATGAATGCCCTGACCTATACCTATACGCGCCAGCATTTTGCAGAGGTCATGAAATCAGTAAATGAAGACCACGTTCCCGT
GGTGGTGACCAGCCAGCGCGGCAAACCGGTTGTCATCCTTTCCCTCGACGATTTCCACGCTTATGAAGAAACCGCCTATC
TGCTCCGTAATCCCCAAGGGGCCAAACGGTTGCTTGAATCTGTGGAGGAACTGCGTGCAGGCGGAGGGCAGACCAGAGAC
CTGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References