Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 740503..741278 Replicon chromosome
Accession NZ_CP074116
Organism Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KGB32_RS03695 Protein ID WP_004150910.1
Coordinates 740793..741278 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KGB32_RS03690 Protein ID WP_004150912.1
Coordinates 740503..740796 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB32_RS03670 (KGB32_03670) 735711..736313 - 603 WP_062954968.1 short chain dehydrogenase -
KGB32_RS03675 (KGB32_03675) 736411..737322 + 912 WP_004188550.1 LysR family transcriptional regulator -
KGB32_RS03680 (KGB32_03680) 737323..738471 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KGB32_RS03685 (KGB32_03685) 738482..739858 - 1377 WP_004217775.1 cystathionine beta-synthase -
KGB32_RS03690 (KGB32_03690) 740503..740796 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KGB32_RS03695 (KGB32_03695) 740793..741278 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KGB32_RS03700 (KGB32_03700) 741982..742575 + 594 WP_004188553.1 hypothetical protein -
KGB32_RS03705 (KGB32_03705) 742672..742888 + 217 Protein_729 transposase -
KGB32_RS03710 (KGB32_03710) 743494..744366 + 873 WP_004188557.1 ParA family protein -
KGB32_RS03715 (KGB32_03715) 744366..744749 + 384 WP_004150906.1 hypothetical protein -
KGB32_RS03720 (KGB32_03720) 744742..746109 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742672..742824 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T201476 WP_004150910.1 NZ_CP074116:740793-741278 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T201476 NZ_CP098447:1846790-1847314 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT201476 WP_004150912.1 NZ_CP074116:740503-740796 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT201476 NZ_CP098447:1846515-1846793 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References