Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1640559..1641334 Replicon chromosome
Accession NZ_CP026177
Organism Klebsiella pneumoniae strain KPNIH50

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag C2U50_RS12465 Protein ID WP_009308645.1
Coordinates 1640559..1641044 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag C2U50_RS12470 Protein ID WP_004150912.1
Coordinates 1641041..1641334 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U50_RS12435 1635727..1637094 - 1368 WP_040236167.1 replicative DNA helicase -
C2U50_RS12440 1637087..1637470 - 384 WP_040236166.1 hypothetical protein -
C2U50_RS12445 1637470..1638342 - 873 WP_040236165.1 ParA family protein -
C2U50_RS12455 1638949..1639165 - 217 Protein_1675 transposase -
C2U50_RS12460 1639262..1639855 - 594 WP_004188553.1 hypothetical protein -
C2U50_RS12465 1640559..1641044 - 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
C2U50_RS12470 1641041..1641334 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
C2U50_RS12475 1641979..1643355 + 1377 WP_072269297.1 cystathionine beta-synthase -
C2U50_RS12480 1643366..1644514 + 1149 WP_040236164.1 PLP-dependent aspartate aminotransferase family protein -
C2U50_RS12485 1644515..1645426 - 912 WP_004181308.1 LysR family transcriptional regulator -
C2U50_RS12490 1645524..1646126 + 603 WP_032448122.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1586780..1641585 54805
flank IS/Tn - - 1639013..1639165 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T93638 WP_009308645.1 NZ_CP026177:c1641044-1640559 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T93638 NZ_CP026177:c1641044-1640559 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT93638 WP_004150912.1 NZ_CP026177:c1641334-1641041 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT93638 NZ_CP026177:c1641334-1641041 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References